Screening and identification of yeasts from passion fruit peels wine with low methanol content

Screening and identification of yeasts from passion fruit peels wine with low methanol content

Blog Article

In this study, yeasts were isolated from the soil of passion fruit Airliner Model Kits orchard, fresh passion fruit, natural fermentation broth of passion fruit and rotten passion fruit by traditional culture separation method.The yeast strains suitable for fruit wine brewing were obtained by preliminary screening of alcohol production ability, gas production performance, fermentation ability and tolerance analysis.Through the determination of methanol and ethanol content, the low-yield methanol-producing yeast strains suitable for the fermentation of passion fruit peel wine were obtained.The screened strains were identified by morphological observation and molecular biology techniques.

The results showed that a total of 22 yeast strains were isolated, of which 8 strains were suitable for fruit wine brewing.Among them, the methanol contents of Party Games passion fruit peel wine fermented by strains gp0, gp2 and gp3 were less than 400 mg/L, which were 163.95 mg/L, 192.04 mg/L and 202.

98 mg/L, respectively, and the ethanol contents were 5.46%vol, 6.38%vol and 5.86%vol, respectively.

The three strains were all identified as Saccharomyces cerevisiae.